Exploring the potential of Cinnamomum zeylanicum oil against drug resistant Helicobacter pylori-producing cytotoxic genes (2024)

J Appl Biomed 20:22-36, 2022|DOI:10.32725/jab.2022.003

Exploring the potential of Cinnamomum zeylanicum oil against drug resistant Helicobacter pylori-producing cytotoxic genes

Sameh S. Ali^{1, 2, *}, Manar K. Abd Elnabi^{1, 2}, Mohammad M. Alkherkhisy³, Abdulkarim Hasan⁴, Fanghua Li⁵, Maha Khalil⁶, Jianzhong Sun^{1, *}, Nessma El-Zawawy²

¹Biofuels Institute, School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang, 212013, China

²Botany Department, Faculty of Science, Tanta University, Tanta, 31527, Egypt

³Department of Microbiology and Immunology, Faculty of Medicine, Al-Azhar University, Cairo, Egypt

⁴Department of Pathology, Faculty of Medicine, Al-Azhar University, Cairo, Egypt

⁵State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin, Heilongjiang Province 150090, China

⁶Biology Department, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia

Thirty-one of sixty dyspeptic patients tested positive for Helicobacter pylori colonization in this study, as determined by histopathology and 16S rRNA. The cytotoxin-associated gene A (cagA) and vacuolating cytotoxin A (vacA) genes were found in 67.7 and 93.5% of H. pylori patients, respectively. The cagA gene was found to be associated with 100% of patients with duodenal erosion and ulceration identified via endoscopy examination. In addition, 86.7% of patients with cancerous and precancerous lesions, glandular atrophy, and intestinal metaplasia identified via histopathology examination. The vacA s1m1 mutation was associated with more severe forms of gastric erosion and ulceration, as well as the presence of precancerous and cancerous lesions. Eighteen (64.3%) of the twenty-eight isolates were classified as multi-drug resistant (MDR) or pan-drug resistant (PDR) H. pylori. Due to a resurgence of interest in alternative therapies derived from plants as a result of H. pylori resistance to the majority of commonly used antibiotics, the inhibitory activity of five essential oils extracted from some commonly used medicinal plants was evaluated in vitro against drug-resistant H. pylori clinical isolates. Cinnamomum zeylanicum essential oil demonstrated the highest anti-H. pylori activity when compared to the other essential oils tested. Cinnamaldehyde was the most abundant compound in C. zeylanicum (65.91%). The toxicological evaluation established the safety of C. zeylanicum oil for human use. As a result, C. zeylanicum essential oil may represent a novel antibacterial agent capable of combating drug-resistant H. pylori carrying cytotoxin genes.

Keywords: Antimicrobial agents; Cinnamaldehyde; Cinnamomum zeylanicum; Cytotoxin-associated genes; Drug-resistance; Helicobacter pylori

Grants and funding:

Foundation of China (31772529). It was also supportedby Taif University Researchers Supporting Project number(TURSP-2020/95), Taif University, Taif, Saudi Arabia.

Conflicts of interest:

All authors declare no competing interests.

Received: October 19, 2021; Revised: January 10, 2022; Accepted: February 15, 2022; Prepublished online: February 16, 2022; Published: March 17, 2022 Show citation

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